Indirect analysis of corticosteroids. I. The determination of 17-hydroxycorticosteroids.
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چکیده
منابع مشابه
Spectrophotometric Determination of Corticosteroids and Its Application in Pharmaceutical Formulation
A simple, sensitive and economically viable spectrophotometric method for the determination of corticosteroids (predinsolone {PSL}, dexamethasone {DEX}, prednisone {PS}, betamethasone sodium phosphate {BEP} and hydrocortisone {HYD} has been developed. The method involves the oxidation of corticosteroids by iron (III) and subsequent complexation of iron (II) with potassium hexacynoferrate (III),...
متن کاملA note on the rapid assay of 17-ketogenic steroids in urine.
The determination of urinary corticosteroids by their conversion to 17-ketosteroids (17-KS) on treatment of urine with sodium bismuthate has been recently reported (Norymberski, 1952; Norymberski, Stubbs, and West, 1953). The group of corticosteroids thus determined has been termed 17-ketogenic steroids (17-KGS). The interest in this method shown by colleagues from other laboratories prompts us...
متن کاملA Modified Procedure for the Estimation of Total 17-hydroxycorticosteroids (porter-silber Chromogens) in Urine.
A modified procedure for the determination of urinary 17-hydroxycorticosteroids(dihydroxy-acetonyl-corticoids) is described. The use of ethyl ether in a single extraction step and the stabilization of the phenylhydrazine-sulfuric acid reagent with sodium chloride are the unique features of the method. Studies of chromogenicities of steroids, recovery, pH effect on extraction, and salt effect on...
متن کاملCefoxitin interferes with the "Clini-Skreen" column method for urinary 17-hydroxycorticosteroids.
Cefoxitin interferes with determination of urinary 17-hydroxycorticosteroids. The apparent concentration of hormone is increased from three- to 10-fold in samples from patients receiving cefoxitin when the Amberlite XAD-2 "Clini-Skreen" column is used. To determine the mechanism of interference, we reacted aqueous solutions of cefoxitin, cortisol, cortisone, and 11-deoxycortisol with phenylhydr...
متن کاملComparison of results by four different procedures for determination of 17-hydroxycorticosteroids.
A Porter and Silber procedure, a fluorometric procedure, a double-isotope derivative procedure, and a competitive protein-binding assay procedure were used to determine 17-hydroxycorticosteroids in plasma from normal individuals and from patients with endocrinopathies. Results from each of these procedures were intercompared. Results of the fluorometric procedure and the competitive proteinbind...
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عنوان ژورنال:
- The Biochemical journal
دوره 60 3 شماره
صفحات -
تاریخ انتشار 1955